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Department of Biochemistry

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https://ir2.lirauni.ac.ug/handle/123456789/369

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    Aflatoxins in Uganda: An Encyclopedic Review of the Etiology, Epidemiology, Detection, Quantification, Exposure Assessment, Reduction, and Control
    (International Journal of Microbiology, 2020) Omara, Timothy; Nassazi, Winfred; Omute, Tom; Aburu, Awath; Laker, Fortunate; Kalukusu, Raymond; Musau, Bashir; Nakabuye, Brenda Victoria; Kagoya, Sarah; Otim, George; Adupa, Eddie
    Uganda is an agrarian country where farming employs more than 60% of the population. Aflatoxins remain a scourge in the country, unprecedentedly reducing the nutritional and economic value of agricultural foods. *is review was sought to synthetize the country’s major findings in relation to the mycotoxins’ etiology, epidemiology, detection, quantification, exposure assessment, control, and reduction in different matrices. Electronic results indicate that aflatoxins in Uganda are produced by Aspergillus flavus and A. parasiticus and have been reported in maize, sorghum, sesame, beans, sunflower, millet, peanuts, and cassava. *e causes and proliferation of aflatoxigenic contamination of Ugandan foods have been largely due to poor pre-, peri-, and postharvest activities, poor government legislation, lack of awareness, and low levels of education among farmers, entrepreneurs, and consumers on this plague. Little diet diversity has exacerbated the risk of exposure to aflatoxins in Uganda because most of the staple foods are aflatoxin-prone. On the detection and control, these are still marginal, though some devoted scholars have devised and validated a sensitive portable device for on-site aflatoxin detection in maize and shown that starter cultures used for making some cereal-based beverages have the potential to bind aflatoxins. More efforts should be geared towards awareness creation and vaccination against hepatitis B and hepatitis A to reduce the risk of development of liver cancer among the populace.
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    Antivenin plants used for treatment of snakebites in Uganda: ethnobotanical reports and pharmacological evidences
    (Tropical Medicine and Health, 2020) Omara, Timothy; Kagoya, Sarah; Openy, Abraham; Omute, Tom; Ssebulime, Stephen; Kiplagat, Kibet Mohamed; Bongomin, Ocident
    Snakebite envenomation is a serious public health concern in rural areas of Uganda. Snakebites are poorly documented in Uganda because most occur in rural settings where traditional therapists end up being the first-line defense for treatment. Ethnobotanical surveys in Uganda have reported that some plants are used to antagonize the activity of various snake venoms. This review was sought to identify antivenin plants in Uganda and some pharmacological evidence supporting their use. A literature survey done in multidisciplinary databases revealed that 77 plant species belonging to 65 genera and 42 families are used for the treatment of snakebites in Uganda. The majority of these species belong to family Fabaceae (31%), Euphorbiaceae (14%), Asteraceae (12%), Amaryllidaceae (10%) and Solanaceae (10%). The main growth habit of the species is shrubs (41%), trees (33%) and herbs (18%). Antivenin extracts are usually prepared from roots (54%) and leaves (23%) through decoctions, infusions, powders, and juices, and are administered orally (67%) or applied topically (17%). The most frequently encountered species were Allium cepa, Carica papaya, Securidaca longipedunculata, Harrisonia abyssinica, and Nicotiana tabacum. Species with global reports of tested antivenom activity included Allium cepa, Allium sativum, Basella alba, Capparis tomentosa, Carica papaya, Cassia occidentalis, Jatropa carcus, Vernonia cinereal, Bidens pilosa, Hoslundia opposita, Maytensus senegalensis, Securinega virosa, and Solanum incanum. There is need to identify and evaluate the antivenom compounds in the claimed plants.
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    Breast‑feeding practices and maternal employment in health facilities of Lira District, Northern Uganda
    (Bulletin of the National Research Centre, 2022) Omute, Tom; Kirungi, Gloria
    Background: In Uganda, many working mothers stop breast-feeding before the end of the mandatory maternity leave of 60 days. Inadequate breast-feeding is a risk factor for infant morbidity and mortality. Thus, understanding of the factors that influence breast-feeding is essential so as to advocate for the appropriate breast-feeding practices. This cross-sectional study assessed the pre-lacteal feeding (PLF), early initiation of breast-feeding (EIBF) and exclusive breast-feeding (EBF) and the associated factors for PLF, EIBF and EBF among working mothers in health facilities in Lira District, Northern Uganda. Results: Among 376 mothers who participated, the prevalence of PLF, EIBF and EBF was 23.2%, 67.0% and 43.9%, respectively. Mode of delivery was significantly associated with PLF (AOR = 0.39, 95% CI 0.22–0.68, p < 0.01), while mode of delivery (AOR = 3.77, 95% CI 2.19–6.47, p < 0.01), length of daily working time (AOR = 0.49, 95% CI 0.29–0.82, p < 0.01) and paid maternity leave (AOR = 0.45, 95% CI 0.22–0.95, p < 0.05) were the statistically significant factors associated with EIBF. Age-group, income level, mode of delivery (AOR = 0.26, 95% CI 0.09–0.74, p < 0.05), distance to workplace (AOR = 0.45, 95% CI 0.26–0.78, p < 0.01), paid maternity leave (AOR = 0.45, 95% CI 0.22–0.95, p < 0.01), breast-feeding space (AOR = 0.30, 95% CI 0.16–0.59, p < 0.05) and breast-feeding break (AOR = 0.84, 95% CI 0.47–1.50, p < 0.01) were the significant factors associated with EBF. Conclusions: Exclusive breast-feeding and pre-lacteal feeding among working mothers in health facilities in Lira District are lower than the national averages, but prevalence of early initiation of breast-feeding is higher than the national average.
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    Helicobacter pylori from Peptic Ulcer Patients in Uganda Is Highly Resistant to Clarithromycin and Fluoroquinolones: Results of the GenoType HelicoDR Test Directly Applied on Stool
    (BioMed Research International, 2017) Angol, Denish Calmax; Ocama, Ponsiano; Kirabo, Tess Ayazika; Okeng, Alfred; Najjingo, Irene; Bwanga, Freddie
    Background. Around 70–90% of peptic ulcer disease (PUD) is due to Helicobacter pylori and requires treatment with antimicrobials to which these bacteria are susceptible. Common H. pylori diagnostic tests do not provide drug susceptibility data. Using the GenoType HelicoDR PCR test designed for gastric biopsies for simultaneous detection of H. pylori and its resistance to clarithromycin (CLA)/fluoroquinolones (FLQ), we present evidence for stool as an optional test specimen and also provide data on prevalence of H. pylori resistance to CLA and FLQ in Uganda. Methods. Stool from 142 symptomatic PUD patients at three hospitals in Kampala was screened for H. pylori using a rapid antigen test.The GenoType HelicoDR test was run on all H. pylori antigen positives to determine PCR positivity and resistance to CLA/FLQ. Results. Thirty-one samples (22%) were H. pylori antigen positive, and 21 (68%) of these were H. pylori PCR positive. Six of the 21 (29%) were resistant to CLA and eight to FLQ (42%), while two gave invalid FLQ resistance results. Conclusion. Stool is a possible specimen for the GenoType HelicoDR test for rapid detection of H. pylori and drug resistance. In Uganda, Helicobacter pylori is highly resistant to CLA and FLQ.
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    Prevalence and Antimicrobial Susceptibility Pattern of Extended Spectrum Beta Lactamase Producers in Gram-negative Urine Isolates at MBN Clinical Laboratories, Kampala Uganda
    (Archives of Microbiology & Immunology, 2018) Kasango, Simon Dembe; Lutoti, Stephen; Wewedru, Izale; Aboce, Emmanuel; Angol, Denish Calmax
    Introduction: Occurrence of Extended Spectrum beta-lactamase (ESBLs) producing bacteria have presented impediment in treatment choices for urinary tract infections. ESBLs embody a major cluster of lactamases accountable for resistance to novel generations of ß-lactam drugs worldwide. The study determined prevalence of ESBL organisms in urine isolates and susceptibility patterns to 13 antibacterial agents. Materials and methods: Two hundred samples were cultured on blood agar, MacConkey agar and incubated at 37°C utmost 48 hours. Isolates identified based on standard bacteriological culture and biochemical characteristics. Drug susceptibility centered on Clinical Laboratory Standard Institute recommended and WHO modified Kirby- Bauer disc diffusion methods. Isolates with reduced susceptibility to Ceftazidime were considered to be possible ESBL producers. Phenotypically confirmed ESBL required use of Ceftazidime in combination with Clavulanic acid. A five milimeter increase zone diameter for Ceftazidime in combination with Clavulanic acid versus its zone tested alone was considered as ESBL. Results: Out of 200 samples, 45 (22.5%) had significant growth, majority Escherichia coli 28 (62.2%), Klebsiella pneumonae 11 (24.4%) followed and Citrobacter fruendii 2 (4.4%). Enterobacter species, Morganella morganii, Proteus mirabilis and Seratia marcescens each 1 (2.2%). Prevalence of ESBLs was 56%. Out of 25 (56%) ESBLs, highest prevalence was among Escherichia coli (15/25; 60%) followed by Klebsiella pneumonae (5/25; 24%) while Citrobacter fruendii, Enterobacter species, Morganella morganii and Proteus mirabilis each had (1/25; 4%). Susceptibility was highest to Imipenem 22 (88%) and least to Ampicillin, Amoxicillin-Clavulanic acid and Tetracycline each 1 (4%). No susceptibility to Cefuroxime and Ceftazidime was observed. Conclusion: The study showed that resistant ESBLs generating bacteria were present among urine isolates. We recommend that ESBLs bacteria isolated in urine be treated based on antibiotics susceptibility, continuous surveillance to guide correct treatment for urinary tract infection and to prevent the occurrence of multi drug resistant bacteria. This should be reflected in the policies developed by the Ministries of health to promote rational use of antibiotics.
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    Safety, Toxicological and Allergenic Aspects of Using Algae for Food
    (Springer, 2024) Kyarimpa, Christine; Omute, Tom; Nakiguli, Caroline K.; Khanakwa, Alice V.; Angiro, Christopher; Kahwa, Ivan; Ahumuza, Fortunate; Omara, Timothy
    Consumption of algae has been historically practiced, especially in East Asia and the Pacific region cultures. However, sporadic events and empirical studies have suggested that some compounds could be triggering intoxications, allergic reactions and mortalities in humans who consumed algae. This chapter is an effort to explore with in-depth attention the safety, toxicological and allergic reactions following human consumption of algae. Based on retrieved literature, it is clear that toxicities and allergies from ingestion of algae are not a rarity, and to date, at least seventy (70) illnesses, six (6) allergic reactions and fourteen (14) mortalities have been reported globally. Toxicities and mortalities from intake of edible algae has been reported in species of Gracilaria, Caulerpa and Acanthophora genus, and are associated with their bioaccumulation of contaminants such as excess iodine, heavy metals, cyanotoxins or toxic inherent compounds such as caulerpenyne, manauealides A and C, prostaglandin E2, polycavernosides, aplysiatoxins and their derivatives. Allergenicity has been reported in Arthrospira, Chlorella, Chondrus, Eucheuma, Gigartina and Palmaria species, with the sulfated polysaccharide (carrageenan) and the photosynthetic pigment (C-phycocyanin) being the implicated allergens. These allergic reactions are mediated through activation of innate immune pathways of inflammation that trigger NF-kB activation, modification of gut microbiota and thickness of mucus barrier. We contend that appropriate labelling of algae-derived food products, public education, proper cleaning of fresh algae before consumption and profiling of toxic and allergenic algal species and compounds could aid in reducing intoxications and allergic reactions from algae used in food and food products. Future studies should consider examining edible algae for contaminants of emerging concern such as microplastics, cyanotoxins, emerging per- and polyfluoroalkyl substances, pharmaceutical residues and personal care products. Keywords: Safety, Toxicological, Allergenic Aspects, Algae, and Food